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2013•Zhongguo xin yao zazhiRequires access

HPLC fingerprint of Sophora flavescens Ait

Liang Jing

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Abstract

Objective: To research the HPLC fingerprint of Sophora flavescens Ait in order to provide a reliable method to evaluate the quality of Sophora flavescens effectively.Methods: The components of Sophora flavescens were separated on a Phenomenex Luna(2) C18 column(250 mm×4.6 mm,5 μm) with linear gradient elution using a mobile phase consisting of acetonitrile and 0.1% aqueous ammonia at a flow rate of 1.0 mL·min-1.The detection wavelength was set at 210 nm and the column temperature was 30 ℃.Results: The HPLC fingerprint was established by testing 12 batches of samples.Fourteen peaks were determined as the common peaks and four of them were identified as oxymartrine,oxysophocarpine,sophoridine and martrine.Conclusion: HPLC fingerprint shows good repeatability.It can be used as a mean to assess and control the quality of Sophora flavescens.

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What this paper is about

Objective: To research the HPLC fingerprint of Sophora flavescens Ait in order to provide a reliable method to evaluate the quality of Sophora flavescens effectively.Methods: The components of Sophora flavescens were separated on a Phenomenex Luna(2) C18 column(250 mm×4.6 mm,5 μm) with linear gradient elution using a mobile phase consisting of acetonitrile and 0.1% aqueous ammonia at a flow rate of 1.0 mL·min-1.The detection wavelength was set at 210 nm and the column temperature was 30 ℃.Results: The HPLC fingerprint was established by testing 12 batches of samples.Fourteen peaks were determined as the common peaks and four of them were identified as oxymartrine,oxysophocarpine,sophoridine and martrine.Conclusion: HPLC fingerprint shows good repeatability.It can be used as a mean to assess and control the quality of Sophora flavescens.

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Available abstract

Objective: To research the HPLC fingerprint of Sophora flavescens Ait in order to provide a reliable method to evaluate the quality of Sophora flavescens effectively.Methods: The components of Sophora flavescens were separated on a Phenomenex Luna(2) C18 column(250 mm×4.6 mm,5 μm) with linear gradient elution using a mobile phase consisting of acetonitrile and 0.1% aqueous ammonia at a flow rate of 1.0 mL·min-1.The detection wavelength was set at 210 nm and the column temperature was 30 ℃.Results: The HPLC fingerprint was established by testing 12 batches of samples.Fourteen peaks were determined as the common peaks and four of them were identified as oxymartrine,oxysophocarpine,sophoridine and martrine.Conclusion: HPLC fingerprint shows good repeatability.It can be used as a mean to assess and control the quality of Sophora flavescens.

Key concepts: Sophora flavescens, Chromatography, Repeatability, High-performance liquid chromatography, Sophora, Gradient elution, Fingerprint (computing), Chemistry

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