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2008•Journal of Hebei North UniversityRequires access

Studies on Analyzing of HPLC Fingerprint for Sophora Flavescens Ait

Jianhua Tang

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Abstract

Objective:To establish a method for analyzing HPLC fingerprints of Sophora flavescens Ait.Methods:The HPLC method was used with Hypersil C18 column(250mm×4.6mm,5μm).The mobile phases were consisted of methanol-acetonitrile-20mmol/L KH2PO4 solution(adjusted pH to 8.0 with triethylamine) respectively,gradient elute.The flow rate was 0.8mL·min-1.The temperature of column was 30℃.The UV detection wavelength was 220nm.Results:The mutual mode of HPLC fingerprints was set up and the similar degrees to the crude drugs of different habitats were compared.Conclusion:The method was simple andreliable,and it was capable of effectively controlling the quality of Sophora flavescens Ait.

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What this paper is about

Objective:To establish a method for analyzing HPLC fingerprints of Sophora flavescens Ait.Methods:The HPLC method was used with Hypersil C18 column(250mm×4.6mm,5μm).The mobile phases were consisted of methanol-acetonitrile-20mmol/L KH2PO4 solution(adjusted pH to 8.0 with triethylamine) respectively,gradient elute.The flow rate was 0.8mL·min-1.The temperature of column was 30℃.The UV detection wavelength was 220nm.Results:The mutual mode of HPLC fingerprints was set up and the similar degrees to the crude drugs of different habitats were compared.Conclusion:The method was simple andreliable,and it was capable of effectively controlling the quality of Sophora flavescens Ait.

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Available abstract

Objective:To establish a method for analyzing HPLC fingerprints of Sophora flavescens Ait.Methods:The HPLC method was used with Hypersil C18 column(250mm×4.6mm,5μm).The mobile phases were consisted of methanol-acetonitrile-20mmol/L KH2PO4 solution(adjusted pH to 8.0 with triethylamine) respectively,gradient elute.The flow rate was 0.8mL·min-1.The temperature of column was 30℃.The UV detection wavelength was 220nm.Results:The mutual mode of HPLC fingerprints was set up and the similar degrees to the crude drugs of different habitats were compared.Conclusion:The method was simple andreliable,and it was capable of effectively controlling the quality of Sophora flavescens Ait.

Key concepts: Sophora flavescens, Chromatography, High-performance liquid chromatography, Gradient elution, Triethylamine, Chemistry, Fingerprint (computing), Sophora

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