Effect of hepatitis B virus genotypes on mutations of hepatitis B virus precore and basic core promotor

Objective To study the effect of hepatitis B virus(HBV) genotypes on mutations of HBV precore and basic core promotor (BCP). Methods Serum samples of 416 patients with chronic hepatitis B(CHB) were determined for precore and BCP mutations by microfluidic chip. Results Among from 416 cases, the genotype of HBV have been determined in 406 cases. The rate of genotype B was 20.9%, genotype C was 65.9%, B and C mix-type was 10.8%, 10 patients's genotypes were not identified. Among 302 HBeAg negative cases, 248 (82.12%)had mutations of precore or/and BCP. The rates of cases with precore mutant BCP mutant and double mutant were 41.06%,31.02% and 9.94%,respectively. The rate of precore mutant in the patients with genotype B was 22.9%,which was significant difference (P0.01) compared with that in patients with genotype C or B and C mix-type(39.4% and 40.0%,respectively). The rates of BCP mutant and two mutants in patients with genotype C were higher than those in patients with genotype B,but no significantly different (P0.05). The rates of precore and/or BCP mutants in patients with B and C mix-type were similar to that in patients with genotype C. Conclusion Hepatitis B virus genotypes may have effect on mutations of precore and/or BCP,especially genotype C.