UV Radiation and Screen for β-carotene-rich Strain of Dunaliella
Guangfa Liu
Abstract
Guangfa Liu
Abstract
The strains containing high content of β-carotene were obtained after the detection of 7 species(13 strains) of Dunaliella deposited in our laboratory.One of the strains was selected and radiated by UV.Experiment results demonstrated that the orange-red colonies of algae could be used as the preliminary marker for screening β-carotene-rich cell.A mutant,dominated as 11-1,containing β-carotene as high as 9.48%(dried mass) was selected after a serious of high salt concentration,high light intensity cultivation and lower nitrogen source conduction.Meanwhile the size of mutant 11-1 was 5 times more than its original pattern.It was propitious to cultivation and collection of the β-carotene hyper-producing new strains of Dunaliella.
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The strains containing high content of β-carotene were obtained after the detection of 7 species(13 strains) of Dunaliella deposited in our laboratory.One of the strains was selected and radiated by UV.Experiment results demonstrated that the orange-red colonies of algae could be used as the preliminary marker for screening β-carotene-rich cell.A mutant,dominated as 11-1,containing β-carotene as high as 9.48%(dried mass) was selected after a serious of high salt concentration,high light intensity cultivation and lower nitrogen source conduction.Meanwhile the size of mutant 11-1 was 5 times more than its original pattern.It was propitious to cultivation and collection of the β-carotene hyper-producing new strains of Dunaliella.
Key concepts: Dunaliella, Carotene, Strain (injury), Orange (colour), Food science, Botany, Mutant, Biology