Optimization of extraction of peanut protein with ultrasonic-assisted aqueous enzymatic methods by response surface methodology
Zongyuan Han
Abstract
Zongyuan Han
Abstract
Based on single factor experiment,the amount of complex enzymes,the optimum ultrasonic pre-irradiation temperature,time,enzyme hydrolysis pH,enzyme hydrolysis temperature and reaction time were chosen as influencing factors and the peanut protein yield was selected as response value.The optimum extraction conditions were as follows:amount of complex enzymes 1.59%,enzyme hydrolysis temperature 56.5℃,reaction time 3.9h,the ratio of material to liquid 1∶ 4.4 and enzyme hydrolysis pH 9.0.The peanut protein yield was 94.31%±0.37%.
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Based on single factor experiment,the amount of complex enzymes,the optimum ultrasonic pre-irradiation temperature,time,enzyme hydrolysis pH,enzyme hydrolysis temperature and reaction time were chosen as influencing factors and the peanut protein yield was selected as response value.The optimum extraction conditions were as follows:amount of complex enzymes 1.59%,enzyme hydrolysis temperature 56.5℃,reaction time 3.9h,the ratio of material to liquid 1∶ 4.4 and enzyme hydrolysis pH 9.0.The peanut protein yield was 94.31%±0.37%.
Key concepts: Yield (engineering), Hydrolysis, Response surface methodology, Extraction (chemistry), Enzymatic hydrolysis, Chemistry, Chromatography, Enzyme