2005•Zhongguo redai yixueRequires access

Determination of content of astragalin from oral compound astragalus soup by using HPLC-ELSD

SU Yu-ya

Abstract

Obiective To develop a method to determine Astragaloside IV in compound Radix Astragali oral solution by HPLC-ELSD. Methods HPLC-ELSD was used for analyzing Astragaloside IV in compound Radix Astragali oral solution, with Alltech C 18 column(5μm, 250×4.6mm),and acetonitrile-water(36:64)as a mobile phase. The flow rate was 1. Oml/min. The temperature of drift tube for ELSD was 110℃ and the flow rate of nebulizing gas was 2.3L/min. Results The calibration curve was liner in the range of 0. 997～15.95μg ,R=0. 9994. The recvery of the added sample was 95.4% and RSD was 4.2%. Conclusion The method is simple, rapid, more precision, accurate and reproducible, and it provides an effective way for quality control of Astragaloside IV in compound Radix Astragali oral solution.

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Available abstract

Key concepts: Drift tube, Radix (gastropod), Chromatography detector, Chromatography, High-performance liquid chromatography, Astragaloside, Chemistry, Calibration curve