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2007•Zhongguo shouyi kexueRequires access

Preparation and identification of monoclonal antibodies against VP1 protein of porcine encephalomyocarditis virus

Zha Zhen-lin

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Abstract

Five 8-weeks-old BALB/c mice were immunized with purified recombinant fusion protein VP1 of porcine encephalomyocarditis virus(EMCV).Two hybridoma cell lines designated 10B and 11D steadily secreting monoclonal antibody(McAb) against VP1 protein of EMCV were obtained by hybridoma technology.The titers of the two McAbs in the supernatant culture supernatant were 1∶1600 and(1∶800,) respectively,and the titers in the ascites of the mice were 1∶2.56×10~(6)and 1∶1.28×10~(6),(respectively,) detected by ELISA.The two McAbs belonged to IgG2a subclass and recognized different epitopes of VP1.The McAbs were able to recognize the recombinant VP1 protein of EMCV by Western-blotting,and could react with EMCV by indirect immunofluorescence assay(IFA) and immunohistoche-(mistry,) indicating that the two McAbs exhibit good specificity.

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Five 8-weeks-old BALB/c mice were immunized with purified recombinant fusion protein VP1 of porcine encephalomyocarditis virus(EMCV).Two hybridoma cell lines designated 10B and 11D steadily secreting monoclonal antibody(McAb) against VP1 protein of EMCV were obtained by hybridoma technology.The titers of the two McAbs in the supernatant culture supernatant were 1∶1600 and(1∶800,) respectively,and the titers in the ascites of the mice were 1∶2.56×10~(6)and 1∶1.28×10~(6),(respectively,) detected by ELISA.The two McAbs belonged to IgG2a subclass and recognized different epitopes of VP1.The McAbs were able to recognize the recombinant VP1 protein of EMCV by Western-blotting,and could react with EMCV by indirect immunofluorescence assay(IFA) and immunohistoche-(mistry,) indicating that the two McAbs exhibit good specificity.

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Available abstract

Five 8-weeks-old BALB/c mice were immunized with purified recombinant fusion protein VP1 of porcine encephalomyocarditis virus(EMCV).Two hybridoma cell lines designated 10B and 11D steadily secreting monoclonal antibody(McAb) against VP1 protein of EMCV were obtained by hybridoma technology.The titers of the two McAbs in the supernatant culture supernatant were 1∶1600 and(1∶800,) respectively,and the titers in the ascites of the mice were 1∶2.56×10~(6)and 1∶1.28×10~(6),(respectively,) detected by ELISA.The two McAbs belonged to IgG2a subclass and recognized different epitopes of VP1.The McAbs were able to recognize the recombinant VP1 protein of EMCV by Western-blotting,and could react with EMCV by indirect immunofluorescence assay(IFA) and immunohistoche-(mistry,) indicating that the two McAbs exhibit good specificity.

Key concepts: Monoclonal antibody, Recombinant DNA, Titer, Virology, Immunofluorescence, Virus, Epitope, Molecular biology

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Preparation and identification of monoclonal antibodies against VP1 protein of porcine encephalomyocarditis virus — Research Paper | ScholarLens