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2008•Progress in Veterinary MedicineRequires access

Preparation and Identification of Monoclonal Aantibodies Against ORF2-M1 Protein of Swine HEV

Xiang Wen-hua

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Abstract

Five 8-weeks-old Calb/c mice were immunized with purified recombinant fusion protein ORF2-M1of swine Hepatitis E virus.Two hybridoma cell lines designated BC4 and 2A10 steadily secreting monoclonal antibody(McAb)against ORF2-M 1 protein of swine Hepatitis E virus were obtained by hybridoma technology.The titers of the two McAbs in the culture supernatant were 1∶1.60×103 and 1∶0.80×103,respectively,and the titers in the ascites of the mice were 1∶2.56×106 and 1∶1.28×106 respectively,detected by ELISA.The two McAbs were able to recognize different epitopes of ORF2-M1.The McAbs could react with HEV by indirect immunofluorescence assay(IFA),indicating that the two McAbs exhibit good specificity.

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What this paper is about

Five 8-weeks-old Calb/c mice were immunized with purified recombinant fusion protein ORF2-M1of swine Hepatitis E virus.Two hybridoma cell lines designated BC4 and 2A10 steadily secreting monoclonal antibody(McAb)against ORF2-M 1 protein of swine Hepatitis E virus were obtained by hybridoma technology.The titers of the two McAbs in the culture supernatant were 1∶1.60×103 and 1∶0.80×103,respectively,and the titers in the ascites of the mice were 1∶2.56×106 and 1∶1.28×106 respectively,detected by ELISA.The two McAbs were able to recognize different epitopes of ORF2-M1.The McAbs could react with HEV by indirect immunofluorescence assay(IFA),indicating that the two McAbs exhibit good specificity.

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Available abstract

Five 8-weeks-old Calb/c mice were immunized with purified recombinant fusion protein ORF2-M1of swine Hepatitis E virus.Two hybridoma cell lines designated BC4 and 2A10 steadily secreting monoclonal antibody(McAb)against ORF2-M 1 protein of swine Hepatitis E virus were obtained by hybridoma technology.The titers of the two McAbs in the culture supernatant were 1∶1.60×103 and 1∶0.80×103,respectively,and the titers in the ascites of the mice were 1∶2.56×106 and 1∶1.28×106 respectively,detected by ELISA.The two McAbs were able to recognize different epitopes of ORF2-M1.The McAbs could react with HEV by indirect immunofluorescence assay(IFA),indicating that the two McAbs exhibit good specificity.

Key concepts: Hepatitis E virus, Monoclonal antibody, Titer, Virology, Recombinant DNA, Epitope, Molecular biology, Fusion protein

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Preparation and Identification of Monoclonal Aantibodies Against ORF2-M1 Protein of Swine HEV — Research Paper | ScholarLens