Expression and identification of capsid protein of porcine circovirus type 2 by recombinant baculovirus system

The ORF2 gene of Porcine Circovirus type 2(PCV2) deleting N-terminal nuclear localization region was amplified by PCR and cloned into the donor vector pMelBacB of Bac-N-Blue Baculovirus Expression System,and the recombinant plasmid was verified.After homologous recombination of the plasmid with Bac-N-Blue linear DNA,the mixture was transfected into Sf9 insect cells by Cellfectin Reagent,and the recombinant Baculovirus was constructed,which expressed recombinant protein on Sf9 cell.The result of confirming by SDS-PAGE,Western-blot and indirect lmmunofluorescence,indicated the recombinant Cap protein was expressed by Baculovirus system.It provided a substance foundation to test the specific antibody of Porcine Circovirus with Cap protein as detection antigen.