Cloning and Expression Profile of Cold-Responsive Gene OsCR1 in Rice

Many biotic stimuli,such as cold,heat and drought adversely affect plant development.To unders(?)and mechanisms underlying stress tolerance and discover novel stress-tolerant genes in rice(Oryza sativa L.),the Affymetrix 60 K GeneChip Rice Genome Array was used to analyze the whole genome expression of rice Pei'ai 64s under cold, drough and heat stress conditions.We identified a gene OsCRI(Oryza sativa L.cold responsive gene 1,GenBank accession: AK066066),which was highly induced by cold in leaf and panicle at all the developmental stages test.The expression profile of OsCR1 was further confirmed by quantitative real-time PCR analysis.The cDNA of OsCR1 was cl(?)ned by RT-PCR and sequenced.The cDNA is 831 bp in length and contains an open reading frame of 585 bp encoding a protein of 195 amino acid residues with a calculated molecular mass of 21.9 kD and a PI of 5.78.Search in GenBank found that OsCR1 is located in chromosome 10 of rice.The OsCR1 gene sequence was found to have two introns and might have alterative splicing phenomenon.Topology prediction showed that the OsCR1 protein contains two Ser/Thr protein kinase phosphorylation sites.Hydrophobic analysis revealed that the prolein is a hydrophilic protein.Five different stress-responsive cis-acting elements were found in the putative promoter region.Alignment with other proteins re- vealed that OsCR1 encodes a protein of unknown function.