Cloning and Analysis of Stress Responsive Gene OsMsr11 in Rice

To discover new stress tolerance genes,OsMsr11(Oryza sativa L.multiple stress responsive gene 11) was chosen to be cloned and analyzed,based on the analysis of global gene expression profiles of rice Pei'ai 64S under heat,drought and cold stresses by using GeneChip Rice Genome Array(Affymetrix).OsMsr11 was a cold-,drought-and heat-inducible gene,and the expression level was highly up-regulated by cold stress at the heading stage.The cDNA of OsMsr11 was cloned through PCR amplification.Sequence analysis showed that the cDNA consists of an open reading frame of 234 bp,encoding a protein of 77 amino acid residues.The encoded protein contains a signal peptide and may be secreted into the periplasmic space.Known-function domain was not observed in the protein sequence.The OsMsr11 gene lacks intron.Analysis of the putative promoter region identified matches to cis-elements related to stress responses.To analyze its function,the OsMSR11 gene was transferred into rice 9311 by Agrobacterium-mediated method.The obtained data showed that over-expression of OsMsr11 in rice enhanced the tolerance of transgenic plants to salt and reduced their sensitivity to ABA.