Study on Preparation and Properties of Doxorubicin-loaded Chitosan Microspheres

Objective To study the preparation technique and release characteristic of doxorubicin-loaded chitosan microspheres for the intracerebral local administration. Methods Using the liquid paraffin as the oil phase,L-ascorbyl palmitate as the cross linking agent,and span-80 as the emulsifier.Doxorubicin-loaded chitosan microspheres were prepared by emulsion-chemical cross link technique.Dynamic dialysis method was used to determine the releasing characteristic of microspheres in vitro. Results Microspheres(doxorubicin /chitosan =1:9) with a good shape and even particle size distribution were prepared.The average particle diameter was(9.41±2.43) μm with a quantity of drug loading(8.49±0.37)%.The proportion of entrapment was(70.56±4.23)%.The drug release in vitro had a satisfied effect of delayed release. Conclusion The optimized preparation technique is stable and has a high entrapment efficiency,suitable for the preparation of doxorubicin-loaded chitosan microspheres for cerebral administration.