2007•Journal of Jinan UniversityRequires access

Establishment of two-dimensional electrophoresis methods for human epidermis proteome

Xiaoping Wu

Abstract

Aim: To establish two-dimensional electrophoresis methods for human epidermis proteome.Methods: The total proteins of human epidermis were extracted by homogenization and separated by immobilized pH gradient(IPG) strips prior to SDS polyacrylamide gel electrophoresis.The gels were stained with silver nitrate,scanned and then analyzed using PDQuest 7.4 analysis software.Results: With 200 μg of protein loaded on 7 cm pH 5～8 IPG strips,414±19 protein spots were detected in the two-dimensional electrophoresis profiles.The matched spots between gels were 346±31 and the match rate was 83.6%.Conclusion:The two-dimensional electrophoresis profiles of human epidermis proteome were obtained with high resolution and reproducibility.

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Key concepts: Proteome, Immobilized pH gradient, Two-dimensional gel electrophoresis, Homogenization (climate), Chromatography, Electrophoresis, Epidermis (zoology), Silver nitrate