Establishment of a two-dimensional gel electrophoresis map of human gastric mucosa proteome

Objective Two-dimensional gel electrophoresis(2-DE) is a key technique for proteomics.To analyze the proteome of gastric mucosa and its change in different disease. 2-DE technique is established. Methods A series of important factors, such as sample preparation, electrophoresis parameters, choice of IPG gels, concentration of SDS gels, protocol for staining were optimized to improve the resolution and reproducibility. Results Using the proper method described above, choosing appropriate sample amount, using pre-cast IPG dry strip and casting 13% equal gel, satisfactory 2-DE maps were obtained.