Study on change of heat shock-related factors in rat hippocampal CA1 region during ischemic brain injury

Objective To clear the expressing of HSP72 mRNA and HSP72 protein in rat hippocampal CA1 region during ischemic brain injury.Methods SD rats were divided randomly into 8 groups:sham-operated,cerebral ischemia and reperfusion 30 min,cerebral ischemia and reperfusion 3 h,cerebral ischemia and reperfusion 12 h,cerebral ischemia and reperfusion 1 d,cerebral ischemia and reperfusion 3 d,cerebral ischemia and reperfusion 5 d,cerebral ischemia and reperfusion 7 d.The expressing of HSP72 mRNA and HSP72 protein in hippocampus of rats was detected by PT-PCR and immunoblot assay at different time of reperfusion.Results There was no any expressing of HSP72 mRNA and HSP72 protein in sham group,some starting from cerebral ischemia and reperfusion 3 h group,peaking in cerebral ischemia and reperfusion 3 d group,and declining in cerebral ischemia and reperfusion 5 d and 7 d groups.Conclusion There is maximal expressing of HSP72 mRNA and HSP72 protein in cerebral ischemia and reperfusion 3 d group although different groups express diversely.