2001•Unpublished venueRequires access
The Change of the Level of Interleukin 8 in Focal Cerebral Ischemia and Ischemia reperfusion Injury in Rats
Cao Qiu
Open publisher page 1 citations
Abstract
Objective To study and compare the role of interleukin 8(IL 8) in cerebral ischemia injury and cerebral ischemia reperfusion injury. Methods The middle cerebral artery(MCA) of rats were occluded by the modified Zea Longa′s technique for different time or followed by reperfusion for different time.IL 8 was detected with double antibody sandwich indirect ELISA of injured brain tissue and blood sera of the two groups. Results The content of IL 8 in injured brain tissue in cerebral ischemia reperfusion group was higher than that in cerebral ischemia group.They both changed time dependently.The former peaked at 22 hours after reperfusion,and then decreased rapidly.The latter peaked at 6 hours after ischemia,and then decreased slowly.The concentration of IL 8 in serum peaked at 1 hour after reperfusion, and decreased rapidly in ischemia reperfusion group.While in ischemia group, it peaked at 3 hours after ischmia,and then decreased slowly. Conclusions IL 8 is involved in both ischemia reperfusion injury and ischemia injury. The damage caused by IL 8 in cerebral ischemia reperfusion injury is more severe than in cerebral ischemia injury.