The Experimental Study of Autologous Red Bone Marrow and the Different Composite Components PCPC Repairing Bone Defect

Objective To discuss the efficacy treatment by autologous red bone marrow(ARBM)and various components composite porous calcium phosphate cement(PCPC)bone defect in order to find the evident of autologous red bone marrow healing role in the bone defect. Through this study we provides experimental evidence for the clinical treatment about bone defects red bone marrow transplant. Method We select 60 healthy New Zealand white rabbits, weighing 2.5~3.0 kg. All animals were established experimental model of bilateral bone defect(1.5 cm). All animals were completely random divided into A, B, C, D, E according to forelimbs(24 side per five groups), which E was the control group. A group was implanted autologous red bone marrow in the bone defect site. Group B was implanted bone marrow mononuclear cells. Group C was implanted composite acellular components; D group was implanted composite bone marrow supernatant cell components; E group was implanted with simple PCPC. we get the samples 4、8 and 12 weeks in each group at each time point and six rabbits were randomly selected(three killed, three under anesthesia)to get X-ray exam, the killed three rabbits receive histological examination such as various periods of new bone formation. We make statistical analysis according to modified Lane-Sandhu X scoring and histological score. Result The gross anatomy, x-ray and histological observation at all time points showed that the bone cells, trabecular bone matrix, bone remodeling, and PCPC degradation inside the implant, ARBM the composite of artificial inoculation(PCPC)group was better than the other groups. Lane-Sandhu X ray and histological score has statistically significant difference(P0.05). ConclusionAutologous red bone marrow and growth factors have the effect on promoting bone defects as well as a synergistic effect in promoting bone defect repair process. Autologous red bone marrow can be used in the treatment of bone defect repair which the main-promoting ingredients was the bone marrow mononuclear cells.