2011•Medical RecapitulateRequires access

Preparation of the Rabbit-anti-human TGF-β_1 Polyclonal Antibody

Chu Yan-hui

Abstract

Objective To prepare the rabbit-anti-human TGF-β1 polyclonal antibody from the immune rabbit with human TGF-β1 recombinant protein.Methods Equal volume of Freund′s adjuvant and recombinant protein were mixed,then New Zealand white rabbits were immunized with the the recombinant protein as antigen.The immune sera of rabbits were collected.Then antibody was purified by ammonium sulfate fractionation,then the polyclonal antibodies titer was detected by the indirect Enzyme-linked immunosorbent assay.Western-blot techniques was used to identify antibody specificity.Results The rabbit-anti-human TGF-β1 polyclonal antibody were successfully obtained.Purified rabbit anti-human polyclonal antibodies titer is 1∶ 10 000.Conclusion The specificity of rabbit anti-human TGF-β1 polyclonal antibodies is better to meet the need of further experiments.

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Key concepts: Polyclonal antibodies, Antibody, Recombinant DNA, Western blot, Molecular biology, Antibody titer, Titer, Immune system