Protection effects of hydroxysafflor yellow A on proliferation and apoptosis of human umbilicalvein endothelial cells

Objective To establish the cell model of restenosis in vitro induced by LPC and investi-gate the effects of HYSA on the protection and treatment of the restenosis1.Methods Human umbilical vein endothelial cells(HUVECs) were cultured in vitro.The study was designated to 4 groups:(1) control group,(2)LPC group,(3)Low-concentration HYSA(50 mg/L) group,(4)high-concentration HYSA(100 mg/L) group.Proliferation and apoptosis of HUVECs were assessed by MTT assay,flow cytometry(FCM) and electron microscope.The nitricoxide(NO) concentration were determned by nitrate reductase assay.respectively.Results Compared with the control group,LPC could inhibit the growth and induce apoptosis of HUVECs as well as decrease NO production in HUVECs.HYSA could increase the proliferation and decrease the apoptosis of HUVECs,and also enhance NO production of HUVECs which induced by LPC.Conclusion HYSA could improve proliferation and reduce apoptosis and enhance NO production of HUVECs through lysophosphatidylcholine,which may be an important route for HYSA to prevent and treat the restenosis after percutaneous coronary intervention.