Effects of OXA and DDP on cell proliferation and Bcl-2,Bax,Caspase-3 expression of BGC-823 cells in vitro

Aim:To observe the effects of OXA and DDP on proliferation and Bcl-2,Bax,Caspase-3 expression of gastric carcinoma cell BGC-823.Methods:The inhibition rate of BGC-823 cells was examined by MTT assay after being treated with OXA(3.125,6.250,12.500,25.000 mg/L),DDP(1.562 5,3.125 0,6.250 0,12.500 0 mg/L)and OXA combined with DDP(3.125/1.562 5,6.250/3.125 0,12.500/6.250 0,25.000/12.500 0 mg/L).Bcl-2,Bax,Caspase-3 expression of BGC-823 cell treated with OXA combined wtih DDP was detected by immunohistochemistry assay.Results:The proliferation inhibitive rate of BGC-823 cells was in time-and dose-dependent manner by OXA,DDP single or combined(P0.01). Compared with single groups,the IC50 of OXA combined with DDP to BGC-823 cells was higher(P0.01). The combined use of OXA and DDP caused Bcl-2 up-regulation [(37.4±2.4)% vs (73.7±2.2)%] and Bax [(56.4±0.7)% vs (19.9±0.7)%],Caspase-3 [(40.9±1.6)% vs (9.5±1.6)%] low-regulation(P0.001).Conclusion:The proliferation of BGC-823 could be inhibited by OXA,DDP single and combined. The combination induces cell apoptosis through mitochondrial pathway.