Role of As_2S_2 on C13K/DDP Cells Proliferation and Apoptosis in vitro

Objective To investigate the role of As2S2 on C13K/DDP cells proliferation and apoptosis in vitro.Methods C13K/DDP cells were incubated with different concentration of As2S2(4,6,8,10μmol/L)at various periods(24,48,72h).The cell growth was measured by MTT.Apoptosis was detected by double staining flow cytometry(FCM).The expression of BCL-2,BAX and AKT was examined by Western blot analysis.Results Compared with DDP group,the proliferation of C13K/DDP cells treated with As2S2 was significantly inhibited in dose-and time-dependent manner(P0.01).FCM analysis showed that As2S2could markedly induce C13K/DDP cells apoptosis.The apoptotic rates of C13K/DDP cells treated with As2S2(6,8μmol/L)after 24h and 48h were(16.05±2)%,(22.30±3)%and(28.94±1.8)%,(37.85±3)%respectively,there was significant difference compared to control group[(7.82±1.2)%,(9.80±2.6)%]and DDP group[(9.45±2)%,(14.74±3.2)% ](P0.05).BCL-2 and AKT expression was down-regulated by As2S2and BAX expression was up-regulated by As2S2.Conclusion As2S2could inhibit the proliferation of C13K/DDP cell and induce cell apoptosis,which may be related to the BCL-2 or AKT down-regulation and BAX up-regulation.