Genetic Relationship Analysis of 38 Punica granatu L. Cultivars Based on RAPD Markers

Taking 38pomegranate cultivars from China germplasm nursery at Yicheng of Zaozhuang as materials,they were amplified by 20primers of RAPD,and the cluster analysis and principal coordnate analysis based on amplification results,the genetic relationship of 38cultivars of the material were studied.The results showed that total 146bands were amplified from 38cultivars,in which 109bands were polymorphic.The percentage of polymorphic band was 74.6%.The genetic similarities coefficient between cultivars ranged from 0.45to 1.0pomegranate cultivars were divided into four groups at 0.58based on UPGMA method.The clustering analysis results did not show obvious correlation with the morphologic classification and the geographical distribution.38pomegranate cultivars were divided into two groups based on the principal coordinates analysis,the results of the principal coordinates analysis were not consistent with that of the UPGMA cluster.