Comparison of either cisplatin or oxaliplatin with hyperthermia against human gastric cancer cell line BGC-823

Objective:To compare the anticancer effect of cisplatin or oxaliplatin combined with hyperthermia against human undifferentiated gastric adenocarcinoma cell line BGC-823. Methods:The working concentrations of cisplatin and oxaliplatin against BGC-823 were determined by MTT assay that has been developed for quantitative evaluation of the proliferation of cells. Then the chemotherapy or hyperthermia, singly or concurrently, were used and the cell survival rates were obtained. The anticancer effect was evaluated and compared by Veleriote method at 24 h or 48 h. Results:The working concentrations were defined as the concentration causing 20%～30% inhibition rate. They turned out to be 1μg/ml and 5μg/ml of cisplatin and oxaliplatin, respectively. Exposing to 43℃ hyperthermia for 1 hour did not show obvious inhibition to BGC-823 at 24 h or 48 h(P0.05). The chemotherapies with cisplatin 1μg/ml or oxaliplatin 5μg/ml did not exhibit obvious inhibition to BGC-823 at 24 h (P0.05), but they did at 48 h(P 0.01). The combination of cisplatin or oxaliplatin with 43℃ hyperthermia for 1 hour showed obvious inhibition to BGC-823 at 24 h and 48 h.(P0.05). And the effect of chemohyperthermia was amplified as the time prolonged(P0.05). There was no difference between the two drugs at 24 h and 48 h when used singly. The inhibitory effect of cisplatin was much higher than that of the oxaliplatin when used with hyperthermia. Judged with the Veleriote method at 24 h and 48 h, there was obvious synergism of cisplatin 1μg/ml combined with hyperthermia. Oxaliplatin combined with hyperthermia exhibited subaddictive effect at 24 h and synergism at 48 h. Conclusion:The combination of cisplatin or oxaliplatin and 43℃ hyperthermia for 1 hour!showed subaddictive effect or synergism to the inhibition of human undifferentiated gastric adenocarcinoma cell line BGC-823,and cisplatin is much better than oxaliplatin when combined with hyperthermia.