Empirical study of different extraction methods influence on the antioxidation of polysaccharide in cordyceps militaris

Objective: To clear the Cordyceps polysaccharide extraction yield and the impact of antioxidation with the three methods, boiling water bath, microwave and ultrasonic extraction methods. Methods: With three different methods, we extract the polysaccharide. extracted by alcohol precipitation, decolorizing, removed protein to make the fine polysaccharide. phenol-sulphate acid to get the polysaccharide content, judged by the ability of removing the hydroxyl radical to measure the antioxidation, then compared with the two results. Results: When the concentration of polysaccharide is 1.0 mg/mL, the impact of polysaccharide antioxidation with boiling water bath and microwave extraction were 95.36% and 97.11%, the result of ultrasonic extraction is worst, just 74.46 percent. Conclusion: The polysaccharide extracted by microwave has the better antioxidation, and the process we would be with a shorter time, and have a higher rate.