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2011•Chinese Journal of Industrial MedicineRequires access

Role of glutamate transporters in neurotoxicity induced by rotenone

XV Zhong-hua

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Abstract

Objective To explore the role of glutamate transporters in the neurotoxicity induced by rotenone.Methods Astrocytes isolated from newborn rats,were cocultured with PC12 cells,then were divided into 6 groups:control group,rotenone treated group,DHK pretreated group(Ⅰ and Ⅱ),and PDC pretreated group(Ⅰ and Ⅱ).Extracellular glutamate concentrations were detected by high performance liquid chromatography(HPLC),and the uptake ability of glutamate was determined with isotope labeling method.Results It was showed that the glutamate uptake ability in astrocytes pretreated with PDC was significantly decreased compared with rotenone treated group,while those in astrocytes pretreated with DHK failed to show any significant change.Conclusion GLAST rather than GLT-1 may play a crucial role in excitotoxicity induced by rotenone.

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Objective To explore the role of glutamate transporters in the neurotoxicity induced by rotenone.Methods Astrocytes isolated from newborn rats,were cocultured with PC12 cells,then were divided into 6 groups:control group,rotenone treated group,DHK pretreated group(Ⅰ and Ⅱ),and PDC pretreated group(Ⅰ and Ⅱ).Extracellular glutamate concentrations were detected by high performance liquid chromatography(HPLC),and the uptake ability of glutamate was determined with isotope labeling method.Results It was showed that the glutamate uptake ability in astrocytes pretreated with PDC was significantly decreased compared with rotenone treated group,while those in astrocytes pretreated with DHK failed to show any significant change.Conclusion GLAST rather than GLT-1 may play a crucial role in excitotoxicity induced by rotenone.

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Available abstract

Objective To explore the role of glutamate transporters in the neurotoxicity induced by rotenone.Methods Astrocytes isolated from newborn rats,were cocultured with PC12 cells,then were divided into 6 groups:control group,rotenone treated group,DHK pretreated group(Ⅰ and Ⅱ),and PDC pretreated group(Ⅰ and Ⅱ).Extracellular glutamate concentrations were detected by high performance liquid chromatography(HPLC),and the uptake ability of glutamate was determined with isotope labeling method.Results It was showed that the glutamate uptake ability in astrocytes pretreated with PDC was significantly decreased compared with rotenone treated group,while those in astrocytes pretreated with DHK failed to show any significant change.Conclusion GLAST rather than GLT-1 may play a crucial role in excitotoxicity induced by rotenone.

Key concepts: Rotenone, Neurotoxicity, Glutamate receptor, Excitotoxicity, Chemistry, Excitatory amino-acid transporter, Transporter, Extracellular

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