2010•Industrial health and Occupational DiseasesRequires access
Effects of Enhancing the Gap Junction on Metabolism of Glutamate in Rotenone Exposed Astrocytes
Hui Liu, Yin FangQiu, Yuhui Zhang, Zhonghua Xu, Gao Qiuju, Jianmei Zhang
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Abstract
Objective To evaluate the effects of anti-arrhythmic peptide 10(AAP-10)and activator of gap junction on metabolism of glutamate in rotenone exposed astrocytes.Methods MTT assay was used to evaluate the toxicity of rotenone on astrocytes,the level of extracellular glutamate was determined by HPLC,the uptake of glutamate was detected with labeled isotope,and the expression of GLAST mRNA was observed by RT-PCR.Results Compared with 1.0 μmol/L rotenone intoxication group,the viabilities of cell culture were significantly increased and the levels of extracellular glutamate were obviously decreased.The function of glutamate uptake and the expression of GLAST mRNA were enhanced in AAP-10 group(250 nmol/L).Conclusions Enhancement of gap junction could significantly ameliorate the disorder of glutamate metabolism in astrocytes induced by rotenone,which might be related to the change of GLAST.