Study on differentially expressed genes of gastric adenocarcinoma by cDNA microarray

OBJECTIVE:Despite the increase in our knowledge of oncogenes and tumor suppressor genes associated with gastric cancer over the past decades, the mechanism of the process of multistage carcinogenesis is still unknown for gastric cancer. The study was designed to investigate the changed gene expression patterns in the gastric carcinogenesis and screening the gastric cancer related genes would provide a credible key to improve the dignosis and therapy of gastric cancer.METHODS:Differently expressed genes of gastric adenocarcinoma in 6 cases were investigated by using high density cDNA microarray representing 18 000 cDNA clones.RESULTS:The genes whose ratios of T/N (gastric adenocarcinoma/normal gastric mucosa) were higher than 2.0 or lower than 0.5 were screened out after hybridization.The expression level of 103 genes differed between gastric adenocarcinoma and normal mucosa in 3 or more samples (one gene differed in all samples ). There were 47 downregulated and 56 upregulared genes in gastric adenocarcinoma.CONCLUSIONS:cDNA microarray technique is effective in screening the differentially expressed genes between gastric adenocarcinoma and normal mucosa.The present study represents a global view of gene expression of gastric adenocarcinoma and provides important clues for further study of gastric adenocarcinoma related genes.