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2013•Biotechnology(Faisalabad)Requires access

Screening and Phylogenetic Analysis of a Strain of Bacillus Inhibiting Animal-derived Pathogens

Sun Chao

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Abstract

Objective:Bacillus which can effectively inhibit animal-derived Escherichia coli and Salmonella were screened from the soil of a broiler farm.Method:Strains of target bacillus were isolated by 96-well high-throughput method.Then,stains were identified by 16S rRNA and gyrB gene analysis.Result:(1) Five strains were proved to inhibit pathogens effectively.Most of all,BP-14 was the most effective strain,which inhibited the growth of three strains of E.coli E1,E3,E4 and one strain of S.gallinarum SC1,and the inhibitory rate was over 75%.(2) BP-14 was identified as Bacillus pumilus based on BLAST and phylogenic analysis.Conclusion:A bacillus strain named BP-14 was isolated from the soil of a broiler farm,which presented an inhibition effect on the growth of animal-derived pathogens.And it was classified as Bacillus pumilus.

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Objective:Bacillus which can effectively inhibit animal-derived Escherichia coli and Salmonella were screened from the soil of a broiler farm.Method:Strains of target bacillus were isolated by 96-well high-throughput method.Then,stains were identified by 16S rRNA and gyrB gene analysis.Result:(1) Five strains were proved to inhibit pathogens effectively.Most of all,BP-14 was the most effective strain,which inhibited the growth of three strains of E.coli E1,E3,E4 and one strain of S.gallinarum SC1,and the inhibitory rate was over 75%.(2) BP-14 was identified as Bacillus pumilus based on BLAST and phylogenic analysis.Conclusion:A bacillus strain named BP-14 was isolated from the soil of a broiler farm,which presented an inhibition effect on the growth of animal-derived pathogens.And it was classified as Bacillus pumilus.

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Available abstract

Objective:Bacillus which can effectively inhibit animal-derived Escherichia coli and Salmonella were screened from the soil of a broiler farm.Method:Strains of target bacillus were isolated by 96-well high-throughput method.Then,stains were identified by 16S rRNA and gyrB gene analysis.Result:(1) Five strains were proved to inhibit pathogens effectively.Most of all,BP-14 was the most effective strain,which inhibited the growth of three strains of E.coli E1,E3,E4 and one strain of S.gallinarum SC1,and the inhibitory rate was over 75%.(2) BP-14 was identified as Bacillus pumilus based on BLAST and phylogenic analysis.Conclusion:A bacillus strain named BP-14 was isolated from the soil of a broiler farm,which presented an inhibition effect on the growth of animal-derived pathogens.And it was classified as Bacillus pumilus.

Key concepts: Bacillus pumilus, Strain (injury), 16S ribosomal RNA, Biology, Bacillus (shape), Microbiology, Escherichia coli, Phylogenetic tree

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