Effect of interferon-gamma on airway inflammation and T lymphoid cells in lung and serum IgE level in murin asthmatic models

[Objective] To investigate the effects of interferon-gamma (IFN-γ) on airway inflammation, T lymphoid cells in lung and serum IgE level in murin asthmatic models. [Methods] C57BL/6 mice were randomly divided into three group: group A (control group, n = 10); group B (asthmatic model group, n = 10); group C (IFN-γ injection group, n = 10). The asthmatic model in group B and C were established by ovalbumin (OVA) and aluminum hydroxide absorbtion. Saline (0.1 ml) and IFN-γ(1 500U) were respectively injected in group B and C on day 1、3、5、9、15、17、20. The cellulal composition of bronch-oalveolar lavage fluid (BALF) on day 22, T lymphoid cells in lung vitro and serum IgE were taken. The cellular composition of BALF, the cytokines secreted IL-4、IL-5 by lung T lymphoid cells and serum IgE level were analised. [Results] The symptoms of group B were more severe than that of group A and C. Group A did not have eosinophil (EOS) in the BALF, while the EOS% in group B and group C was 20.1±7 .0、0.7±0.2、IL-4、IL-5 production from lung T lymphoid cells and IgE level in serum were markedly increased of murin models groups with significant differences between the control healthy mice (P 0.01). IL-4、IL-5 production from lung T lymphoid cells and IgE level in serum were markedly reduced in IFN-7 injection groups with significant differencesbetween the asthmatic model (P0.0l). [Conclusion] IFN-γ could inhibit airway inflammtion in asthmatic mice. One of its mechamism may be that it can inhibitIL-4 and IL-5 prodution from lung T lymphoid cells and reduc IgE level in serum.