2009•中国医师杂志Requires access
The effects of CD4~+ CD25~+ regulatory T cells on the airway inflammation of asthmatic rats
Xue Ke-ying, Chengguo Wang, Li Cheng, Zhongwei Yang, Zhengyan Wang, Wei Li, Ming Shi, Youyong Tang
Open publisher page 0 citations
Abstract
Objective To observe the effects of CD4~+ CD25~+ regulatory T cells ( CD4~+ CD25~+ Treg) on the airway inflammation of asthmatic rats. Methods CD4~+ CD25~+ Treg of OVA- immune tolerance rats were transferred to asthmatic rats. Then bronchoalveolar lavage fluid (BALF) was collected, and cytology study was conducted. The IL-4, IL-5, IFN-γ and OVA-specific serum IgE level in BALF were determined by ELISA. The lung tissue was obtained, and histologieal analysis was done through H. E. Results Total cells number, the percentage of lymphocytes and neutrophils in BALF, the IL-4 and IL-5 BALF levels and the OVA-specific serum IgE level of adoptive transfer group were decreased ( P < 0.05 ) , and the percentage of eosinophils ( Eos) was significantly lower than that of asthma group ( P < 0.01) , while its BALF IFN-γ level was higher than that of asthma group( P <0. 05). Compared with that of asthma group, peribronchiole inflammatory of treated group was alleviated. Conclusion CD4 ~+ CD25~+ Treg of OVA- immune tolerance rats transferred to asthmatic rats can significantly alleviate the airway inflammation of asthmatic rats.
Key words:
CD3-positive T-lymphocytes; T-lymphocytes,helper-inducer; Asthma/PP; Interleukin-4/ME; Interleukin-5/ME; Interferon type Ⅱ