Analysis for a proliferation-inducing ligand gene expression on peripheral blood mononuclear gells from patients with primary biliary cirrhosis

Objective To establish fluorescent relative-quantification polymerase chain reaction for detecting a proliferation-inducing ligand(APRIL)mRNA and to explore the association of its expression level with pathogenesis of primary biliary cirrhosis(PBC).Methods APRIL mRNA of PBMCs in 30 healthy people and 40 PBC patients was measured by means of real-time fluorescent relative-quantification reverse transcriptional PCR.The relative level of APRIL gene expression was calculated by comparison of CT values.Results APRIL mRNA relative level was significantly higher in the patients with PBC(△CT=9.3±2.0)than controls(P0.05.Following analysis of data calculated by the method with 2-△△Ct,the relative expression level of APRIL mRNA in the patients with PBC was 3.5 fold higher than that of control.Eighty percent of PBC patients were positive for ANA among whom 19 were anti-nuclear membrane antibodies and 13 were others.The relative expression levels of APRIL mRNA in the two groups were 6.5 and 1.7 times respectively as high as that of control.Conclusions APRIL mRNA expression level of PBMCs in PBC patients elevated significantly,which was associated with ANA fluorescent patterns.