2010•Zhonghua laonian xin-nao-xueguanbing zazhiRequires access
The protective effect and mechanism of edaravone against myocardium reperfusion injury in rats
Yingying Cong
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Abstract
Objective To investigate the protective effect and mechanism of edaravone against myocardium reperfusion injury in rats.Methods Twenty-four Wistar rats were randomly divided into control group,ischemia-reperfusion group and protective group.After experiment,the activity of CK-MB,GSH-PX and concentration of MDA were detected.The myocardial morphology was observed with light microscope.TUNEL staining and immunohistochemical methods were used to determine apoptosis of myocardial cells and expression of Bcl-2 and Bax proteins.Results The activity of serum CK-MB and MDA were higher and the activity of GSH-PX was lower in ischemiareperfusion group than in control group.The activity of serum CK-MB and content of MDA were lower and the activity of GSH-PX was higher in protective group than in ischemia-reperfusion group.There was large area of myocardial infarction in ischemia-reperfusion group;but the myocardial injury was lighter in protective group.Apoptotic cells was less in protective group than in ischemia-reperfusion group.Bcl-2,Bax protein and the ratio of Bax/Bcl-2 were significantly higher in ischemia-reperfusion group than in control group (P 0.01),the expression of protein Bcl-2 was significantly higher in protective group than in ischemia-reperfusion group (P 0.01),but the expression of protein Bax and ratio of Bax/Bcl-2 were significantly lower in protective group than in ischemia-reperfusion group,and was higher in ischemia-reperfusion group than in control group.Conclusions Edaravone can reduce the extent of myocardial injury,the mechanism is concerned with reducing free radical damage and inhibiting myocardial cell apoptosis.