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A STUDY ON THE OPTIMUM CONDITION OF CGM HYDROLYZDE WITH DIFFERENT PROTEASE TO PRODUCE SOLUBLE PEPTIDE

Sang Liwei

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Abstract

Hydrolys is effect of alkaline protease and neutral protease by single on corn protein was studied.the optimum technology conditions are defined with the indexes Degree of Hydrolysis (DH %).The optimum hydrolyzing conditions for neutral protease have been determined:when pH is 7.5,hydrolysis temperature by protease is 50 °C,the volume of protease is 2 % and the density of substratum is 2.5 %,DH % can be arrived at 24.75 % by 4 hours hydrolysis;The optimum hydrolyzing conditions for alkaline protease have been determined:when pH is 10.0,hydrolysis temperature by protease is 60 ℃,the volume of protease is 1 % and the density of substratum is 1.5 %,DH % can be arrived at 31.01 % by 4 hours hydrolysis.

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Hydrolys is effect of alkaline protease and neutral protease by single on corn protein was studied.the optimum technology conditions are defined with the indexes Degree of Hydrolysis (DH %).The optimum hydrolyzing conditions for neutral protease have been determined:when pH is 7.5,hydrolysis temperature by protease is 50 °C,the volume of protease is 2 % and the density of substratum is 2.5 %,DH % can be arrived at 24.75 % by 4 hours hydrolysis;The optimum hydrolyzing conditions for alkaline protease have been determined:when pH is 10.0,hydrolysis temperature by protease is 60 ℃,the volume of protease is 1 % and the density of substratum is 1.5 %,DH % can be arrived at 31.01 % by 4 hours hydrolysis.

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Available abstract

Hydrolys is effect of alkaline protease and neutral protease by single on corn protein was studied.the optimum technology conditions are defined with the indexes Degree of Hydrolysis (DH %).The optimum hydrolyzing conditions for neutral protease have been determined:when pH is 7.5,hydrolysis temperature by protease is 50 °C,the volume of protease is 2 % and the density of substratum is 2.5 %,DH % can be arrived at 24.75 % by 4 hours hydrolysis;The optimum hydrolyzing conditions for alkaline protease have been determined:when pH is 10.0,hydrolysis temperature by protease is 60 ℃,the volume of protease is 1 % and the density of substratum is 1.5 %,DH % can be arrived at 31.01 % by 4 hours hydrolysis.

Key concepts: Protease, Hydrolysis, Alkaline protease, Chemistry, Neutral protease, Chromatography, Volume (thermodynamics), Enzyme

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A STUDY ON THE OPTIMUM CONDITION OF CGM HYDROLYZDE WITH DIFFERENT PROTEASE TO PRODUCE SOLUBLE PEPTIDE — Research Paper | ScholarLens