Study on Hydrolysis Effects of Neutral Protease and Alkaline Protease on Corn Protein
Liangzhong Liu
Abstract
Liangzhong Liu
Abstract
Hydrolysis effect of alkaline protease and neutral protease by single and compound on corn protein was studied. The optimum hydrolyzing conditions for neutral protease have been determined: [E] 3544 U/g, pH 7.0, temperature 55 ℃ and time(t) 2 h; The optimum hydrolyzing conditions for alkaline protease: [E] 840 U/g, pH 8.0, temperature 55 ℃ and time 2 h. In addition, two protease compound hydrolyzing corn protein is better than the single, and its DH can achieve above 20%.
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Hydrolysis effect of alkaline protease and neutral protease by single and compound on corn protein was studied. The optimum hydrolyzing conditions for neutral protease have been determined: [E] 3544 U/g, pH 7.0, temperature 55 ℃ and time(t) 2 h; The optimum hydrolyzing conditions for alkaline protease: [E] 840 U/g, pH 8.0, temperature 55 ℃ and time 2 h. In addition, two protease compound hydrolyzing corn protein is better than the single, and its DH can achieve above 20%.
Key concepts: Protease, Alkaline protease, Neutral protease, Hydrolysis, Chemistry, Enzyme, Chromatography, Protease inhibitor (pharmacology)