Construction of the Specific Expression Vector of Expressing the Human Lysozyme Gene in Bovine Mammary Gland

The high-fidelity PCR method had been used to amplificate 1.8kb and 1.1kb 5'and 3' regulatory sequences of bovine casein gene in mammary grand.Then it was cloned into TA vector.After sequencing,the recombinant DNA technology had been used to subclone into the modified eukaryotic expression vector pcDNA3(removal of CMV promoter),and insert the human gene lysozyme(hLYZ)of cDNA.The mammary grand-specific expression vector of cattle had been constructed.Verificated by the restriction enzyme digestion and PCR method,the restructuring carrier had succeeded in cloning the area of control casein gene 5 'and 3' and constructing a human lysozyme gene expression system of bovine mammary gland-specific expression vector.