The Cloning of Bovine Beta-lactoglobulin Gene and the Identification of Its Effect for Regulating the Expression of Foreign Gene

Objective To get the regulatory elements which are essential for generating mammary gland bioreactors,and identify their effect for regulating the expression of foreign gene.Methods The whole 8.2 Kb bovine beta-lactoglobulin gene was amplified by PCR method,including the 1.8 Kb 5′ flanking region,4.7 Kb gDNA region,1.7 Kb 3′ flanking region.All the amplified fragments were cloned in T-vectors and were proved to be correct by restrction enzyme digestion and sequencing analysis.The five fragments and the luciferase cDNA were ligated together to get the luciferase temporary expression vector,which was temporarily expressed in the mammary gland of mice.Results The luciferase activity was detected in the milk of mice whose mammary glands were injected with the expression vector.Conclusion The cloned bovine BLG gene could direct foreign gene expression in the mammary gland of mice.\;