Optimization of microwave extraction of polyphenols from malusasiatica pomace by response surface methodology

The polyphenols were extracted from the malusasiatica pomace by ethanol,based on the single factor experimental analysis,the effects of ethanol concentration,material/liquid ratio,microwave power and microwave time on extraction yield of polyphenols were evaluated by response surface analysis methodology(RSM).Based on Box-Behnken experimental design principle,RSM design of three factors and three levels was applied.A regression equation prediction model was established by SAS software for the analysis of response surface values,and the extraction processing parameters were optimized.The results showed that the above four factors reached a significant level on the yield(P0.01),the practical extraction yield was well predicted by the regression model.The optimum extraction conditions were as follows:ethanol concentration was 52%,material/liquid ratio(g/mL)was 1 ∶21,microwave power was 540W and microwave time was 123s.Under these conditions,the extraction yield of malusasiatica pomace polyphenols was 4.27mg/g,which was consistent with the prediction of 97.05%.Compared with solvent extraction,application of microwave increased the yield of polyphenols greatly and also shortened the extraction time.