Optimization of Preparation Process for Hydrolyzed Grass Carp Visceral Proteins without Adding Exogenous Enzymes

Grass carp visceral waste was used as the raw material to prepare hydrolyzed animal proteins by autolysis in an aqueous NaCl medium without the presence of exogenous enzymes.In order to achieve maximum degree of hydrolysis(DH) of grass carp visceral waste,operating conditions such as NaCl amount,material/water ratio,temperature,holding time and pH were optimized using orthogonal array design method.The optimal conditions for maximizing the DH of grass carp visceral waste were material/water ratio 1:1.5,temperature 45 ℃,holding time 4 h,pH 9.0,and as a result,a DH of 77.43% was obtained.