Quantitative determination of flavonoids in red wine by HPLC with fluorescence detector

In order to establish quantitative determination of quercetin,morin hydrate and kaempferol in red wine by high performance liquid chromatography(HPLC),The experiment adopted Polaris C18(2.0×100mm 3μm)at 60℃,mobile phase A of 0.1mol/L HAc and mobile phase B of acetonitrile.Acetonitrile was used for gradient elution at rate of 0.2ml/min,post-column derivation.The detection excitation wavelength was set at 438nm and emission wavelength at 483nm.The results showed that calibration curves were linear at ranges of 0.25mg/L~4.0mg/L,average recovery between 89.7%~95.1% and RSD 1.37%~-2.98%.This method was simple with good reproducibility and can be used as quality control of quercetin,morin hydrate and kaempferol of natural goods.