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Determination of seven kinds of flavone aglycones in red wine with HPLC-UV and their bioactivities

Weinong Zhang

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Abstract

Quantitative determination of quercetin,morin hydrate,kaempferol,isorhamnetin,luteolin,apigenin in red wine with high performance liquid chromatography(HPLC) with ternary gradient was established.The flavonoids were separated by HPLC with Luna C18(4.6mm×150mm,3μm)and a Phenomenex C18 precolumn(4mm×3.0mm) at 50℃.Mobile phase A,B and C were acetonitrile,ethanol and 0.2% H3PO4,respectively.Gradient elution rate was 0.25ml/min and detection wavelength was 265nm.The results showed that the calibration curves were linear at ranges of 0.7mg/L~32.0mg/L.The average recovery in the between of 98.2%~101.1% and RSD 1.88%~2.85%.The method is simple and accurate with good reproducibility and can be used in quantity determination of flavonoid aglycone of natural product.

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What this paper is about

Quantitative determination of quercetin,morin hydrate,kaempferol,isorhamnetin,luteolin,apigenin in red wine with high performance liquid chromatography(HPLC) with ternary gradient was established.The flavonoids were separated by HPLC with Luna C18(4.6mm×150mm,3μm)and a Phenomenex C18 precolumn(4mm×3.0mm) at 50℃.Mobile phase A,B and C were acetonitrile,ethanol and 0.2% H3PO4,respectively.Gradient elution rate was 0.25ml/min and detection wavelength was 265nm.The results showed that the calibration curves were linear at ranges of 0.7mg/L~32.0mg/L.The average recovery in the between of 98.2%~101.1% and RSD 1.88%~2.85%.The method is simple and accurate with good reproducibility and can be used in quantity determination of flavonoid aglycone of natural product.

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Available abstract

Quantitative determination of quercetin,morin hydrate,kaempferol,isorhamnetin,luteolin,apigenin in red wine with high performance liquid chromatography(HPLC) with ternary gradient was established.The flavonoids were separated by HPLC with Luna C18(4.6mm×150mm,3μm)and a Phenomenex C18 precolumn(4mm×3.0mm) at 50℃.Mobile phase A,B and C were acetonitrile,ethanol and 0.2% H3PO4,respectively.Gradient elution rate was 0.25ml/min and detection wavelength was 265nm.The results showed that the calibration curves were linear at ranges of 0.7mg/L~32.0mg/L.The average recovery in the between of 98.2%~101.1% and RSD 1.88%~2.85%.The method is simple and accurate with good reproducibility and can be used in quantity determination of flavonoid aglycone of natural product.

Key concepts: Chemistry, Kaempferol, Chromatography, Isorhamnetin, High-performance liquid chromatography, Apigenin, Wine, Quercetin

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