Effect of Matrine Modification X on Apoptosis of Human Nasopharyngeal Carcinoma CNE1 Cells

Objective:To investigate the effects and mechanism of matrine modification X on inducing apoptosis of human nasopharyngeal carcinoma CNE1 cells in vitro.Method:MTT assay was used to measure the anti-proliferative effect of modification X on CNE1.The cell apoptosis rates and cell cycle were analyzed by flow cytometry.The protein expression of Bax,Bcl-2 and p53 was examined by Western blot.Result:After incubation of CNE1 cells with modification X for 48 h,the modification X could inhibit the proliferation of CNE1 cells with a dose dependent manner.Flow cytometry analysis revealed the apoptosis rate in 58,116 μmol.L-1 modification X group was higher than the matrine group by 70.02% and 75.73% respectively,which showed the significant difference compared with the control group(P 0.05,P 0.01).Compared with the control group,Modification X could significantly arrest the cell cycle at G1 stage(P 0.05,P 0.01).Western blot showed that the expression of Bax and p53 protein significantly increased by modification X in CNE1 cells,at the same time,the expression of Bcl-2 significantly decreased by modification X with a concentration dependent manner(P 0.05,P 0.01).Conclusion:Modification X could induce apoptosis of CNE1 cells in vitro,and the possible mechanism is related to arresting the cell cycle,increasing the Bax and p53 protein expression level and decreasing the Bcl-2 protein expression level.