Effects of electroacupuncture on ET-1,NO content of liver tissue in mouse

Objective: To observe the changes of endothelin-1(ET-1),nitric oxide(NO) content of liver tissue in mouse after electroacupuncture(EA) at different acupoints,and to explore the effects of EA on vasoactive substances in liver and the differences of effects in different acupoints.Methods: The mice were randomly and averagely divided into four groups,EA Zusanli group,EA Ququan group,EA non-acupoint group and control group without EA.After 30min of EA or without EA,NO content in liver tissue of each group was detected by nitric acid reductase;ET-1 content was detected by radioimmunoassay(RIA),and were analyzed and compared.Results: ET-1 content of liver tissue in control group was highest and the next in the non-acupoint group,Ququan group,lowest in Zusanli group.NO content of liver tissue in Zusanli l group was highest and the next in the Ququan group,non-acupoint group,lowest in control group.NO content of Zusanli group was significantly higher than other groups.There were statistically significant differences in the mean ET-1 as the non-acupoint group and control group compared with Zusanli group,and Ququan group compared with control group(P0.05).Conclusion: NO was increased,and ET-1was decreased by EA in liver tissue of healthy mice.It was suggested that acupoint was of specificity in the effects.The effect was strongest in Zusanli group.