Effect of Instant Electroacupuncture on CGRP, ET in Plasma and ETR-A, CGRPmRNA in Colon Tissue of IBS Rat

Objective: To compare with the effect of instant electroacupuncture(EA) at Tianshu and Zusanli points on calcium gene related peptide(CGRP), endothelin(ET) in the plasma and Endothelin receptor A(ETR- A), CGRPmRNA in colon tissue of the irritable bowel syndrome(IBS) model rats so as to discuss the immediate effect of acupuncture. Methods: To divide the young WISTAR rats into blank control group, model group, Tianshu group and Zusanli group, eight per group. The blank control group was untreated, model group was bounded but not given EA, The Tianshu group and Zusanli group were to be given EA in the 8th week once for 20 min. All rats were put to be death after treatment. Taking the plasma and colon tissue of the rats to check the bioactive substance in them. Calcitomin-gene-related peptide(CGRP), endothelin(ET) and endothelin A receptor(ETR-A) were checked by Enzyme immunoassay method. Using RT-PCR medthod to check the expression of CGRPmRNA. Results:(1) Effect of immediate EA at different points with CGRP, ET in plasma in IBS rats: Compared with the blank control group, the level of CGRP reduced significantly in model group(P0. 01); compared with the model group, the level of CGRP increased significantly in Tianshu group and Zusanli group(P0.01). Compared with the blank control group, the level of ET increased significantly in model group(P0.05); compared with the model group, the level of ET reduced significantly in Tianshu group(P0.01).(2) Effect of immediate EA for ETR-A level in colon tissues in IBS rats: compared with the blank control group, the level of ETR-A increased significantly in model group(P0.01); compared with the model group, the level of ETR-A decreased significantly in Zusanli group(P0.01).(3)Effect of immediate EA for CGRP mRNA expression in colon tissue in IBS rats: compared with the blank control group, the expression of CGRPmRNA was enhanced significantly in model group, Tianshu group and Zusanli group(P0.01, P0.05). Compared with the model group, the expression of CGRPmRNA was decreased in Zusanli group(P0.05). Conclusion: Instant electroacupuncture Tianshu point and Zusanli Point can adjust the body's internal environment disorders and CGRP, ETbalance disorders. Tianshu point can adjust the CGRP and ET in the plasma, the effect of Zusanli point express obviously in gene.