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Inhibition of RNA interference on the expression of vascular endothelial growth factor in human ovarian cancer OVCAR-3 cells

Chen Jian-gu

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Abstract

Objectives to investigate the feasibility and specificity of RNA interference which mediated by SiRNA on the gene therapy for ovarian cancer.Methods The VEGF SiRNA was transfected into OVCAR-3 cells to induce RNAi by using transfecting agent.The changes of VEGF gene expression in both siRNA treatment groups and control groups were measured by RT-PCR,ELASA and Western blotting in vitro.Results Experiments in vitro showed that siRNA directly down-regulated the expression of VEGF in the mRNA and protein level(P 0.05).Conclusions RNAi mediated by siRNA markedly decrease VEGF gene expression and may have the potential as a therapeutic modality to treat human ovarian cancer.

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What this paper is about

Objectives to investigate the feasibility and specificity of RNA interference which mediated by SiRNA on the gene therapy for ovarian cancer.Methods The VEGF SiRNA was transfected into OVCAR-3 cells to induce RNAi by using transfecting agent.The changes of VEGF gene expression in both siRNA treatment groups and control groups were measured by RT-PCR,ELASA and Western blotting in vitro.Results Experiments in vitro showed that siRNA directly down-regulated the expression of VEGF in the mRNA and protein level(P 0.05).Conclusions RNAi mediated by siRNA markedly decrease VEGF gene expression and may have the potential as a therapeutic modality to treat human ovarian cancer.

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Available abstract

Objectives to investigate the feasibility and specificity of RNA interference which mediated by SiRNA on the gene therapy for ovarian cancer.Methods The VEGF SiRNA was transfected into OVCAR-3 cells to induce RNAi by using transfecting agent.The changes of VEGF gene expression in both siRNA treatment groups and control groups were measured by RT-PCR,ELASA and Western blotting in vitro.Results Experiments in vitro showed that siRNA directly down-regulated the expression of VEGF in the mRNA and protein level(P 0.05).Conclusions RNAi mediated by siRNA markedly decrease VEGF gene expression and may have the potential as a therapeutic modality to treat human ovarian cancer.

Key concepts: RNA interference, Small interfering RNA, Transfection, Cancer research, Ovarian cancer, In vitro, Gene expression, Messenger RNA

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Inhibition of RNA interference on the expression of vascular endothelial growth factor in human ovarian cancer OVCAR-3 cells — Research Paper | ScholarLens