Development of a Duplex Real-time PCR for Detecting PRRSV

In order to development a real-time PCR method for detecting PRRSV and differentiating general and high pathogenic PRRSV strains,according to PRRSV NSP2 gene sequence in Genbank,a pair of primers and probes were designed.The primers and probes included NSP2 deletion region.Real-time PCR reaction conditions were optimized,use both general and high pathogenic PRRSV strain and other virus.The results showed that the method can be used for detecting and differentiating of PRRSV and high pathogenic PRRSV strains.This method was used to detect clinical samples.28 common strains in 32 samples are positive,the positive rate are 87.5%.17 high pathogenic strains are positive,the positive rate are 59.38%.The established of methods can be used for rapid detection of PRRSV and specific distinction between general and highly pathogenic strains.