CONSTRUCTION OF VP7 GENE OF HUMAN ROTAVIRUS IN PICHIA PASTORIS EXPRES-SION SYSTEM

[Objective] To construct VP7 gene of Human Rotavirus glycoprotein in Pichia pastoris expression system.[Methods] Recombination expression plasmid VP7-pPICZαA was transformed to the competent Pichia pastoris GS115 cells by using electroporation method after being linear by SacⅠ.The recombinant transformants were screened with Zeocin and identified by PCR.Phenotype was identified by using MDH and MMH plate.[Results] The linear recombinant plasmid pPICZαA-VP7 were transformed into competent Pichia pastoris successfully.The recombinant strains were proven correct after identification with PCR,and their phenotypes were Mut+.[Conclusion] The expression system of human rotavirus VP7 gene in Pichia pas-toris was successfully constructed.