High Performance Secretion and Expression of vvIBDV VP2 in Pichia pastoris

The VP2 gene of vvIBDV was inserted into the Pichia pastoris expression vector of pPICZαA. Then the recombinant plasmid of pPICZαA VP2 was transformed into X-33 by electroporation. The multi-copy insertion transformants X-33-pPICZαA-VP2 were screened by PCR 、phenotype and Zeocin-resistance. The expressed VP2 accumulated up to about 0.459mg/mL. SDS-PAGE 、Western blot and animal experiment demonstrated that VP2 product has the similar bioactivity and antigenicity as the natural one.