Genetic diversity analysis of Beauveria bassiana from Magu Mountains of Anhui Province based on SSR molecular marker

In this study,multilocus microsatellite genotyping and genetic diversity of 102 Beauveria bassiana isolates from Magu Mountains of Anhui Province were investigated using SSR(simple sequence repeat) molecular markers.For the sub-populations divided by sampling time,their Nei’s gene diversity(He),Shannon’s diversity index(I),coefficient of gene differentiation(Gst),and gene flow Nm were 0.198 7,0.278 9,0.174 5 and 1.215 0,respectively.However,for the sub-populations grouped based on host orders,the He,I,Gst,and Nm were 0.194 5,0.287 1,0.166 4 and 1.300 0,respectively.The results indicated that there was a certain genetic diversity among B.bassiana isolates from different time and the same geographical origins.Sub-interpopulations showed a low level genetic variation,with 18 isolates belonging to the identical genotype.However,sub-intrapopulations showed a high level genetic variation,and thirty-one multilocus microsatellite genotypes were determined for the 102 B.bassiana isolates using 9 primer sets.Five of the 31 genotypes possessed a higher level of relative dominance.The anniversary genotypes dynamics revealed that the five multilocus microsatellite genotypes continued to theirs spread and epidemicity in the local Masson’s pine plantation ecosystem by infecting different host.However,these relative dominant genotypes were not evenly distributed among different months,but in most months,only one or two dominant genotypes existed.The study suggested that SSR markers can be used as rapid and strain-specific molecular markers for the population genetics,and genotyping of Beauveria.