Study on the Genetic Diversity of Beauveria bassiana (Bals.) Vuill.in Yunnan

Beauveria bassiana is a broad spectrum of entomopathogenic fungi,it has played an important role in the natural control on pest population.To verify the genetic diversity of B.bassiana strains and enrich the diversity content of B.bassiana in Yunnan,the genetic diversity of 39 B.bassiana strains from different locations and different insect hosts in Yunnan Province was evaluated using the inter-simple sequence repeat(ISSR) markers.Eleven ISSR primers were selected for higher reproducibility and polymorphism.The average of 10 fragments was amplified,and the polymorphic was 99.09%.Nei's genetic diversity(He) of those strains from different host insects was 0.347 8,the total genetic diversity(Ht) was 0.351 8,the genetic diversity(Hs) from intra-populations was 0.326 3,the genetic differentiation(Gst) was 0.072 4;The gene flow(Nm) was 6.404 4 for the sub-population isolates,and it showed that genetic differentation caused by the genetic drift.Nei's genetic diversity(He) of those strains from different host insects was 0.344 4 and Shannon genetic diversity index(I)was 0.515 2,the total genetic diversity Ht was 0.347 3,the genetic diversity Hs from intra-populations was 0.213 1,the genetic differentiation Gst was 0.386 2.Overall,all the above results indicated that there was an complicated genetic diversity and higher genetic heterogeneity,and a low degree of genetic differentiation was in the B.bassiana among populations.