Cloning and expression of the gene coding for Mycobacterium tuberculosis antigen CFP10

To clone and express the gene coding for Mycobacterium tuberculosis antigen CFP10,and to establish a basis for diagnosis of tuberculosis,the gene coding CFP10 was amplified by polymerase chain reaction(PCR),and then the gene was inserted to vector pET-30a(+)to construct the recombinant plasmid.The recombinant plasmid was transformed into expressive vector E.coli BL21(DE3),and was induced with IPTG.The presence of recombinant protein in the expression vector was analyzed by SDS-PAGE.The recombinant CFP10 protein was expressed in inclusion body in E.coli BL21(DE3),and the target gene had been cloned into host bacterium.These results would establish a basis for diagnosis of tuberculosis.